Asociación entre polimorfismos del gen de adiponectina y estado nutricional en escolares de la comuna de Hualpén.

Gloria Orellana, Jorge Sapunar, Katia Sáez, Claudio Aguayo, Carlos Calvo, Claudia Radojkovic, Benilde Riffo, Andrea Gleisner, Sylvia Asenjo, Natalia Ulloa

Resumen


ASSOCIATION BETWEEN ADIPONECTIN GENE POLYMORPHISMS AND OBESITY IN SCHOOL AGE CHILDREN FROM HUALPÉN, CHILE

Background: Several genetic polymorphisms of adiponectin have been associated to metabolic diseases as obesity and co-morbidities. Aim: To investigate if there are associations between +45TG, +276GT, -11,377CG y -11,391GA adiponectin SNPs (single nucleotide polymorphism) with obesity in a Chilean children population. Material and methods: A case-control study was performed in 241 obese and 126 normal weight children (7-11 years old) from the urban community of Hualpén, BioBio region. Children were classified as normal or obese, according  to age and gender-specific percentiles defined by Center for Disease Control and Prevention (CDC). The analysis of serum markers was carried out using commercial kits. Adiponectin polymorphisms were determined through a High Resolution Melting (HRM)-enabled real time PCR and by DNA fragment sequencing. Results: The observed allelic frequencies of the studied SNPs were over 11%. The 11,377CG polymorphism was associated with a high risk of obesity, calculated by the additive inheritance model (odds ratio = 1.389, 95% confidence interval: 1.001-1.929, p = 0.049). Conclusions: Obese school children of the Bíobío Region, have an increased risk of carrying the susceptibility allele polymorphism 11377CG of adiponectin gene.


Palabras clave


(Rev Med Chile…) (Key-words: apM-1 protein; Child; Obesity; Polymorphisms, Single Nucleotide)